Lincomycin Biosynthesis Involves a Tyrosine Hydroxylating Heme Protein of an Unusual Enzyme Family

0423382 - MBÚ 2014 RIV US eng J - Článek v odborném periodiku
Novotná, Jitka - Olšovská, Jana - Novák, Petr - Mojzes, P. - Chaloupková, R. - Kameník, Zdeněk - Spížek, Jaroslav - Kutejová, Eva - Marečková, M. - Tichý, Pavel - Damborský, J. - Janata, Jiří
Lincomycin Biosynthesis Involves a Tyrosine Hydroxylating Heme Protein of an Unusual Enzyme Family.
PLoS ONE. Roč. 8, č. 12 (2013). ISSN 1932-6203. E-ISSN 1932-6203
Grant CEP: GA MŠMT(CZ) EE2.3.20.0055; GA MŠMT(CZ) EE2.3.30.0003; GA AV ČR IAA500200810; GA MŠMT(CZ) LC06010
Výzkumný záměr: CEZ:AV0Z50200510
Klíčová slova: lincomycin * peroxidases * DOPA
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 3.534, rok: 2013

The gene lmbB2 of the lincomycin biosynthetic gene cluster of Streptomyces lincolnensis ATCC 25466 was shown to code for an unusual tyrosine hydroxylating enzyme involved in the biosynthetic pathway of this clinically important antibiotic. LmbB2 was expressed in Escherichia coli, purified near to homogeneity and shown to convert tyrosine to 3,4-dihydroxyphenylalanine (DOPA). In contrast to the well-known tyrosine hydroxylases (EC 1.14.16.2) and tyrosinases (EC 1.14.18.1), LmbB2 was identified as a heme protein. Mass spectrometry and Soret band-excited Raman spectroscopy of LmbB2 showed that LmbB2 contains heme b as prosthetic group. The CO-reduced differential absorption spectra of LmbB2 showed that the coordination of Fe was different from that of cytochrome P450 enzymes. LmbB2 exhibits sequence similarity to Orf13 of the anthramycin biosynthetic gene cluster, which has recently been classified as a heme peroxidase. Tyrosine hydroxylating activity of LmbB2 yielding DOPA in the presence of (6R)-5,6,7,8-tetrahydro-L-biopterin (BH4) was also observed. Reaction mechanism of this unique heme peroxidases family is discussed. Also, tyrosine hydroxylation was confirmed as the first step of the amino acid branch of the lincomycin biosynthesis
Trvalý link: http://hdl.handle.net/11104/0229478