Involvement of phosphatidylinositol 4,5-bisphosphate in RNA polymerase I transcription

0423030 - ÚMG 2014 RIV GB eng J - Článek v odborném periodiku
Yildirim, Sukriye - Castano, Enrique - Sobol, Margaryta - Philimonenko, Vlada - Dzijak, Rastislav - Venit, Tomáš - Hozák, Pavel
Involvement of phosphatidylinositol 4,5-bisphosphate in RNA polymerase I transcription.
Journal of Cell Science. Roč. 126, č. 12 (2013), s. 2730-2739. ISSN 0021-9533. E-ISSN 1477-9137
Grant CEP: GA ČR GAP305/11/2232; GA ČR(CZ) GD204/09/H084; GA MŠMT LC545; GA MŠMT(CZ) LC06063
Grant ostatní: CONACYT(MX) 176598
Institucionální podpora: RVO:68378050
Klíčová slova: nucleolus * transcription * PIP2 * UBF * fibrillarin
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 5.325, rok: 2013

RNA polymerase I (Pol I) transcription is essential for the cell cycle, growth and protein synthesis in eukaryotes. In the present study, we found that phosphatidylinositol 4,5-bisphosphate (PIP2) is a part of the protein complex on the active ribosomal promoter during transcription. PIP2 makes a complex with Pol I and the Pol I transcription factor UBF in the nucleolus. PIP2 depletion reduces Pol I transcription, which can be rescued by the addition of exogenous PIP2. In addition, PIP2 also binds directly to the pre-rRNA processing factor fibrillarin (Fib), and co-localizes with nascent transcripts in the nucleolus. PIP2 binding to UBF and Fib modulates their binding to DNA and RNA, respectively. In conclusion, PIP2 interacts with a subset of Pol I transcription machinery, and promotes Pol I transcription.
Trvalý link: http://hdl.handle.net/11104/0229191