The Synthesis, Surface Modification and Stability of SPIO Nanoparticles for MRI Application

0422608 - ÚPT 2014 RIV CZ eng C - Konferenční příspěvek (zahraniční konf.)
Kovář, D. - Fohlerová, Z. - Malá, A. - Jiřík, Radovan - Starčuk jr., Zenon - Kalina, M. - Skládal, P.
The Synthesis, Surface Modification and Stability of SPIO Nanoparticles for MRI Application.
NANOCON 2013. 5th International Conference Proceedings. Ostrava: TANGER Ltd, 2013, s. 113. ISBN 978-80-87294-44-4.
[NANOCON 2013. International Conference /5./. Brno (CZ), 16.10.2013-18.10.2013]
Grant CEP: GA MŠMT ED0017/01/01
Institucionální podpora: RVO:68081731
Klíčová slova: nanoparticles * surface modifications
Kód oboru RIV: BH - Optika, masery a lasery

The biocompatibility and biodegradability of new magnetic resonance imaging (MRI) contrast agents is high-ly desired. The superparamagnetic iron oxide (SPIO) nanoparticles are suitable candidates for these pur-poses. Here the co-precipitation technique for synthesis of monodispersed SPIO nanoparticles is pre-sented. The critical point of the synthesis is core formation and consequent crystal growth. The conditions for core formation (time, temperature, rate of base addition) were optimised. The nanoparticles were stabilised by either silanisation or polymer coating (cationic chitosan, poly-D-Arg, dextran, gelatine, hyaluronic acid). The stability was investigated in physiological pH, different ionic strength solutions, PBS-albumine solution or blood plasma. The biocompatibility was tested in vitro either in the presence of Saccharomyces cerevisiae or erythrocytes suspension. The size and shape was investigated by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The molecular structures of nanoparticles were investigated by FTIR (Fourier transform infrared spectroscopy). The iron cations were determined using the Prussian blue staining test. The stability of nano-particles was investigated by the zeta potential using the method of electrophoretic light scattering (ELS).
Trvalý link: http://hdl.handle.net/11104/0228772