Recombinant alpha-L-rhamnosidase of Aspergillus terreus immobilization in polyvinylalcohol hydrogel and its application in rutin derhamnosylation

0422606 - MBÚ 2014 RIV GB eng J - Článek v odborném periodiku
Rebroš, M. - Pilniková, A. - Šimčíková, Daniela - Weignerová, Lenka - Stloukal, R. - Křen, Vladimír - Rosenberg, M.
Recombinant alpha-L-rhamnosidase of Aspergillus terreus immobilization in polyvinylalcohol hydrogel and its application in rutin derhamnosylation.
Biocatalysis and Biotransformation. Roč. 31, č. 6 (2013), s. 329-334. ISSN 1024-2422. E-ISSN 1029-2446
Grant CEP: GA MŠMT(CZ) 7E11010
Institucionální podpora: RVO:61388971
Klíčová slova: alpha-L-rhamnosidase * Aspergillus terreus * Immobilization
Kód oboru RIV: CE - Biochemie
Impakt faktor: 1.093, rok: 2013

Recombinant alpha-L-rhamnosidase from Aspergillus terreus expressed in Pichia pastoris was immobilized in LentiKats (R) lens-shaped polyvinylalcohol (PVA) capsules with an activity of 7 U g(-1), which was 21% of its original activity. Immobilization did not significantly affect the pH and temperature profile of alpha-L-rhamnosidase, K-M increased by a factor of 3.4 whereas V-max decreased more than 10-fold. No decrease in activity was observed after 27 repeated batch runs of rutin derhamnosylation. The enzyme proved to have an excellent storage stability (136 days) in 60 g L-1 ethanol with no change in its activity
Trvalý link: http://hdl.handle.net/11104/0228875