A telomerase-independent component of telomere loss in chromatin assembly factor 1 mutants of Arabidopsis thaliana

0422265 - BFÚ 2014 RIV DE eng J - Článek v odborném periodiku
Jaške, K. - Mokroš, P. - Mozgová, I. - Fojtová, Miloslava - Fajkus, Jiří
A telomerase-independent component of telomere loss in chromatin assembly factor 1 mutants of Arabidopsis thaliana.
Chromosoma. Roč. 122, č. 4 (2013), s. 285-293. ISSN 0009-5915. E-ISSN 1432-0886
Grant CEP: GA MŠMT(CZ) ED1.1.00/02.0068
Grant ostatní: GA ČR(CZ) GAP501/11/0289
Institucionální podpora: RVO:68081707
Klíčová slova: FACTOR-I * GENOME INSTABILITY * MOLECULAR ANALYSIS
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 3.260, rok: 2013

Dysfunction of chromatin assembly factor 1 in FASCIATA mutants (fas) of Arabidopsis thaliana results in progressive loss of telomeric DNA. Although replicative telomere shortening is typically associated with incomplete resynthesis of their ends by telomerase, no change in telomerase activity could be detected in vitro in extracts from fas mutants. Besides a possible telomerase malfunction, the telomere shortening in fas mutants could presumably be due to problems with conventional replication of telomeres. To distinguish between the possible contribution of suboptimal function of telomerase in fas mutants under in vivo conditions and problems in conventional telomere replication, we crossed fas and tert (telomerase reverse transcriptase) knockout mutants and analyzed telomere shortening in segregated fas mutants, tert mutants, and double fas tert mutants in parallel.
Trvalý link: http://hdl.handle.net/11104/0228466