Catalysis of Hydrogen Evolution by Polylysine, Polyarginine and Polyhistidine at Mercury Electrodes

0422260 - BFÚ 2014 RIV DE eng J - Článek v odborném periodiku
Vargová, Veronika - Živanovic, Marko - Dorčák, Vlastimil - Paleček, Emil - Ostatná, Veronika
Catalysis of Hydrogen Evolution by Polylysine, Polyarginine and Polyhistidine at Mercury Electrodes.
Electroanalysis. Roč. 25, č. 9 (2013), s. 2130-2135. ISSN 1040-0397. E-ISSN 1521-4109
Grant CEP: GA ČR(CZ) GA13-00956S
Výzkumný záměr: CEZ:AV0Z50040702
Institucionální podpora: RVO:68081707
Klíčová slova: CONSTANT-CURRENT CHRONOPOTENTIOMETRY * BOVINE SERUM-ALBUMIN * CARBON ELECTRODES
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 2.502, rok: 2013

Protein catalyzed hydrogen evolution reaction at mercury-containing electrodes controlled by constant-current chronopotentiometric stripping (CPS) is representing a new tool useful in protein research. The resulting CPS peak H is sensitive to changes in the protein structure and its amino acid composition. Besides CPS, cyclic voltammetry appears to be useful for study of poly(amino acids) as an intermediate model system between peptides and macromolecular proteins. Here we show that similarly as arginine in polyarginine and lysine in polylysine also histidine residues in polyhistidine contribute to the catalysis of hydrogen evolution under the given conditions. Peak potentials of individual poly(amino acids) are different and depend on the type of amino acid residues.
Trvalý link: http://hdl.handle.net/11104/0228461