Neonatal colonization of germ-free mice with Bifidobacterium longum prevents allergic sensitization to major birch pollen allergen Bet v 1

0422117 - MBÚ 2014 RIV GB eng J - Článek v odborném periodiku
Schwarzer, Martin - Šrůtková, Dagmar - Schabussova, I. - Hudcovic, Tomáš - Akgün, J. - Wiedermann, U. - Kozáková, Hana
Neonatal colonization of germ-free mice with Bifidobacterium longum prevents allergic sensitization to major birch pollen allergen Bet v 1.
Vaccine. Roč. 31, č. 46 (2013), s. 5405-5412. ISSN 0264-410X. E-ISSN 1873-2518
Grant CEP: GA ČR GA303/09/0449; GA MŠMT 7AMB12AT020
Institucionální podpora: RVO:61388971
Klíčová slova: Allergy * Bifidobacterium * Germ-free mice
Kód oboru RIV: EC - Imunologie
Impakt faktor: 3.485, rok: 2013

The main goal in reversing the allergy epidemic is the development of effective prophylactic strategies. We investigated the prophylactic effect of neonatal mother-to-offspring mono-colonization with Bifidobacterium longum ssp. longum CCM 7952 on subsequent allergic sensitization. Adult male and female germ-free (GF) mice were mono-colonized with B. longum, mated and their offspring, as well as age-matched GF controls, were sensitized with the major birch pollen allergen Bet v 1. Furthermore, signaling pathways involved in the recognition of B. longum were investigated in vitro. Neonatal mono-colonization of GF mice with B. longum suppressed Bet v 1-specific IgE-dependent beta-hexosaminidase release as well as levels of total IgE and allergen-specific IgG2a in serum compared to sensitized GF controls. Accordingly, Bet v 1-induced production of both Thl- and Th2-associated cytokines in spleen cell cultures was significantly reduced in these mice. The general suppression of Bet v 1-specific immune responses in B. longum-colonized mice was associated with increased levels of regulatory cytokines IL-10 and TGF-beta in serum. In vitro, B. longum induced low maturation status of bone marrow-derived dendritic cells and production of IL-10 in TLR2-, MyD88-, and MAPK-dependent manner
Trvalý link: http://hdl.handle.net/11104/0228319