0421945 - ÚEB 2014 RIV GB eng J - Článek v odborném periodiku
Petrovská, Beáta - Jeřábková, Hana - Kohoutová, Lucie - Cenklová, Věra - Pochylová, Žaneta - Gelová, Zuzana - Kočárová, Gabriela - Váchová, Lenka - Kurejová, Michaela - Tomaštíková, Eva - Binarová, Pavla
Overexpressed TPX2 causes ectopic formation of microtubular arrays in the nuclei of acentrosomal plant cells.
Journal of Experimental Botany. Roč. 64, č. 14 (2013), s. 4575-4587. ISSN 0022-0957. E-ISSN 1460-2431
Grant CEP: GA ČR GAP501/12/2333; GA ČR GBP501/12/G090; GA ČR GP204/09/P155; GA ČR(CZ) GA204/07/1169
Institucionální podpora: RVO:61389030 ; RVO:61388971
Klíčová slova: Arabidopsis thaliana * AtTPX2 * Aurora kinase
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 5.794, rok: 2013 ; AIS: 1.695, rok: 2013
DOI: https://doi.org/10.1093/jxb/ert271

TPX2 performs multiple roles in microtubule organization. Previously, it was shown that plant AtTPX2 binds AtAurora1 kinase and colocalizes with microtubules in a cell cycle-specific manner. To elucidate the function of TPX2 further, this work analysed Arabidopsis cells overexpressing AtTPX2-GFP. Distinct arrays of bundled microtubules, decorated with AtTPX2-GFP, were formed in the vicinity of the nuclear envelope and in the nuclei of overexpressing cells. The microtubular arrays showed reduced sensitivity to anti-microtubular drugs. TPX2-mediated formation of nuclear/perinuclear microtubular arrays was not specific for the transition to mitosis and occurred independently of Aurora kinase. The fibres were not observed in cells with detectable programmed cell death and, in this respect, they differed from TPX2-dependent microtubular assemblies functioning in mammalian apoptosis. Colocalization and co-purification data confirmed the interaction of importin with AtTPX2-GFP. In cells with nuclear foci of overexpressed AtTPX2-GFP, strong nuclear signals for Ran and importin diminished when microtubular arrays were assembled. This observation suggests that TPX2-mediated microtubule formation might be triggered by a Ran cycle. Collectively, the data suggest that in the acentrosomal plant cell, in conjunction with importin, overexpressed AtTPX2 reinforces microtubule formation in the vicinity of chromatin and the nuclear envelope.
Trvalý link: http://hdl.handle.net/11104/0228191