Characterization of Neutral Lipase BT-1 Isolated from the Labial Gland of Bombus terrestris Males

0421832 - ÚOCHB 2014 RIV US eng J - Článek v odborném periodiku
Brabcová, Jana - Prchalová, Darina - Demianova, Zuzana - Bučánková, A. - Vogel, H. - Valterová, Irena - Pichová, Iva - Zarevúcka, Marie
Characterization of Neutral Lipase BT-1 Isolated from the Labial Gland of Bombus terrestris Males.
PLoS ONE. Roč. 8, č. 11 (2013), e80066/1-e80066/11. ISSN 1932-6203. E-ISSN 1932-6203
Grant CEP: GA TA ČR TA01020969; GA ČR GA203/09/1446
Institucionální podpora: RVO:61388963
Klíčová slova: lipase * labial gland * Bombus terrestris
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 3.534, rok: 2013
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0080066

This is the first report on neutral lipasefrom the cephalic part of B. terrestris labial glands. Purified lipase BT-1 displays broad specificity but preferentially hydrolyzes shorter fatty acid chains (C8-C12) from diacylglycerols and triacylglycerols. The lipase is most active at neutral pH, with a pH optimum of 8.3. The highest level of lipase BT-1 gene and protein expression is in young individuals. In bumblebees older than 3 days, the lipase gene expression level and enzymatic activity are decreased. Interestingly, according to transcript-level RNA seq analysis, the expression of lipase BT-1 is significantly higher in the bumblebee labial gland than in the fat body. The lipase BT-1 gene is the second most abundantly expressed gene in the labial gland of B. terrestris, which suggests important role of this enzyme in this tissue where pheromones are accumulated.
Trvalý link: http://hdl.handle.net/11104/0228080