H-terminated diamond as optically transparent impedance sensor for real-time monitoring of cell growth

0421495 - FZÚ 2014 RIV DE eng J - Článek v odborném periodiku
Ižák, Tibor - Novotná, Katarína - Kopová, Ivana - Bačáková, Lucie - Rezek, Bohuslav - Kromka, Alexander
H-terminated diamond as optically transparent impedance sensor for real-time monitoring of cell growth.
Physica Status Solidi B. Roč. 250, č. 12 (2013), s. 2741-2746. ISSN 0370-1972. E-ISSN 1521-3951
Grant CEP: GA ČR(CZ) GBP108/12/G108; GA ČR GAP108/12/0996
Institucionální podpora: RVO:68378271 ; RVO:67985823
Klíčová slova: cell cultivation * diamond thin films * impedance measurements * label-free biosensors
Kód oboru RIV: JB - Senzory, čidla, měření a regulace; JJ - Ostatní materiály (FGU-C)
Impakt faktor: 1.605, rok: 2013

Here, we present intrinsic diamond film as a material suitable for fully optically transparent impedance biosensor. Conductive interdigitated electrodes are realized in plane by local hydrogen termination of diamond surface, thereby the surface morphology is uniform. The diamond-based impedance sensors are used for real-time electrical (label-free) monitoring of osteoblast-like cells. Fluorescence images confirm that H/O-termination provides a patterned cell colonization at lower cell seeding concentration. Time-dependent impedance measurements at 1 kHz exhibit similar profiles for the diamond and Au reference sensors. Nevertheless, the diamond sensor seems superior in terms of overall transparency, uniform morphology, and sensitivity to the development of the cell cultures.
Trvalý link: http://hdl.handle.net/11104/0227816