Electrophysiological and imaging methods to study the properties of neurons and glia

0399998 - ÚEM 2014 RIV eng U - Uspořádání akce
Anděrová, Miroslava - Vyklický ml., Ladislav
Electrophysiological and imaging methods to study the properties of neurons and glia.
[Prague, 09.09.2013-11.09.2013, (W-WRD 11/11)]
Institucionální podpora: RVO:68378041 ; RVO:67985823
Klíčová slova: calcium imaging * patch-clamp technique * glie
Kód oboru RIV: FH - Neurologie, neurochirurgie, neurovědy

The aim of the workshop was to train participating students in new methods that allow vizualization of specific cell types in central nervous system, such as expression of fluorescent dyes under the promoter for GFAP, CSPG4 and GLAST (typical markers for astrocytes and polydendrocytes), to train them in methods enabling electrophysiological and morphological characterization of cells in nervous tissue (patch clamp and 3D confocal morphometry) and to employ intracellular calcium imaging and confocal microscopy.
Trvalý link: http://hdl.handle.net/11104/0227335