Počet záznamů: 1  

Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus

  1. 1.
    0399949 - MBÚ 2014 RIV CZ eng J - Článek v odborném periodiku
    Čechová, D. - Nováková, M. - Mikulík, Karel - Novotná, O. - Julák, J. - Zanvit, P. - Prokešová, L.
    Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus.
    Folia Microbiologica. Roč. 58, č. 2 (2013), s. 111-121. ISSN 0015-5632. E-ISSN 1874-9356
    Grant ostatní: GA ČR(CZ) GA310/07/0675
    Institucionální podpora: RVO:61388971
    Klíčová slova: TOXIN-B SUBUNIT * INFLUENZA-VIRUS * INTRANASAL IMMUNIZATION
    Kód oboru RIV: EE - Mikrobiologie, virologie
    Impakt faktor: 1.145, rok: 2013

    Mucosal immunization with non-living antigens usually requires the use of an adjuvant. The adjuvant activity of Bacillus firmus in the mucosal immunization of mice was described by our laboratory previously. In the present study, subcellular localization of B. firmus activities was followed. After mechanical disintegration, subcellular components of bacterium were fractionated by differential centrifugation and salting out. Bacterial cell walls, cytoplasmic membrane fraction, soluble cytoplasmic proteins, and ribosomal fractions were isolated. Their effect on the mouse immune system was studied. Lymphocyte proliferation and immunoglobulin formation in vitro were stimulated by bacterial cell wall (BCW), cytoplasmic membrane (CMF), and ribosomal fractions. BCW and CMF increased antibody formation after intratracheal immunization of mice with influenza A and B viruses, and increased protection against subsequent infection with influenza virus. The BCW fraction even induced intersubtypic cross-protection: Mice immunized with A/California/7/04 (H3N2) + BCW were resistant to the infection by the highly pathogenic A/PR/8/34 (H1N1) virus
    Trvalý link: http://hdl.handle.net/11104/0227126

     
     
Počet záznamů: 1  

  Tyto stránky využívají soubory cookies, které usnadňují jejich prohlížení. Další informace o tom jak používáme cookies.