Počet záznamů: 1  

Syntenic Relationships between the U and M Genomes of Aegilops, Wheat and the Model Species Brachypodium and Rice as Revealed by COS Markers

  1. 1.
    0399936 - ÚEB 2014 RIV US eng J - Článek v odborném periodiku
    Molnár, I. - Šimková, Hana - Leverington-Waite, M. - Goram, R. - Cseh, A. - Vrána, Jan - Farkas, A. - Doležel, Jaroslav - Molnár-Láng, M. - Griffiths, S.
    Syntenic Relationships between the U and M Genomes of Aegilops, Wheat and the Model Species Brachypodium and Rice as Revealed by COS Markers.
    PLoS ONE. Roč. 8, č. 8 (2013). ISSN 1932-6203. E-ISSN 1932-6203
    Grant CEP: GA ČR GBP501/12/G090
    Grant ostatní: GA MŠk(CZ) ED0007/01/01
    Program: ED
    Výzkumný záměr: CEZ:AV0Z50380511
    Klíčová slova: CHROMOSOME ADDITION LINES * IN-SITU HYBRIDIZATION * TRITICUM-AESTIVUM
    Kód oboru RIV: EB - Genetika a molekulární biologie
    Impakt faktor: 3.534, rok: 2013

    Diploid Aegilops umbellulata and Ae. comosa and their natural allotetraploid hybrids Ae. biuncialis and Ae. geniculata are important wild gene sources for wheat. With the aim of assisting in alien gene transfer, this study provides gene-based conserved orthologous set (COS) markers for the U and M genome chromosomes. Out of the 140 markers tested on a series of wheat-Aegilops chromosome introgression lines and flow-sorted subgenomic chromosome fractions, 100 were assigned to Aegilops chromosomes and six and seven duplications were identified in the U and M genomes, respectively. The marker-specific EST sequences were BLAST-ed to Brachypodium and rice genomic sequences to investigate macrosyntenic relationships between the U and M genomes of Aegilops, wheat and the model species. Five syntenic regions of Brachypodium identified genome rearrangements differentiating the U genome from the M genome and from the D genome of wheat. All of them seem to have evolved at the diploid level and to have been modified differentially in the polyploid species Ae. biuncialis and Ae. geniculata. A certain level of wheat-Aegilops homology was detected for group 1, 2, 3 and 5 chromosomes, while a clearly rearranged structure was showed for the group 4, 6 and 7 Aegilops chromosomes relative to wheat. The conserved orthologous set markers assigned to Aegilops chromosomes promise to accelerate gene introgression by facilitating the identification of alien chromatin. The syntenic relationships between the Aegilops species, wheat and model species will facilitate the targeted development of new markers specific for U and M genomic regions and will contribute to the understanding of molecular processes related to allopolyploidization.
    Trvalý link: http://hdl.handle.net/11104/0227069

     
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