Interactions of γ-tubulin with GIT-PIX signaling cassette in mast cells

0399691 - ÚMG 2014 CZ eng A - Abstrakt
Sulimenko, Vadym - Hájková, Zuzana - Sulimenko, Tetyana - Černohorská, Markéta - Dráber, Pavel
Interactions of γ-tubulin with GIT-PIX signaling cassette in mast cells.
Cytoskeletal club 2013. Praha: Československá biologická společnost, 2013.
[Cytoskeletal Club 2013. 15.05.2013-17.05.2013, Vranovská Ves]
Grant CEP: GA MŠMT(CZ) LD13015; GA ČR GPP302/11/P709
Institucionální podpora: RVO:68378050
Klíčová slova: GIT-PIX signaling cassette * Gama-tubulin * Mast cell
Kód oboru RIV: EB - Genetika a molekulární biologie

The lecture presented our recent data of the interaction between γ-tubulin and proteins GIT and PIX in the course of mast cell activation. In general, rapid reorganization of microtubule cytoskeleton during mast cell activation is pivotal as well as signal transduction from surface receptors to centrosome, where microtubules are nucleated. γ-Tubulin is a key regulator of microtubule nucleation. Previously we have shown that γ-tubulin interacts with transiently phosphorylated proteins, some of them were identified as proteins GIT and PIX. These proteins are known to form stable complex called GIT-PIX signaling cassette. Pull-down experiments with truncated forms of γ-tubulin revealed that GIT and PIX proteins interact with the H11-H12 region of the C-terminal part of γ-tubulin. Moreover, these interactions are modulated by calcium ions concentration and by state of microtubule organization. These data suggest new interaction partners on the issue of γ-tubulin regulation in mast cells.
Trvalý link: http://hdl.handle.net/11104/0228399