Epilobium angustifolium extract demonstrates multiple effects on dermal fibroblasts in vitro and skin photo-protection in vivo

0399482 - BFÚ 2014 RIV SK eng J - Článek v odborném periodiku
Ruszová, E. - Cheep, J. - Pavek, S. - Moravcová, M. - Hermanová, M. - Matějková, I. - Spilková, J. - Velebný, V. - Kubala, Lukáš
Epilobium angustifolium extract demonstrates multiple effects on dermal fibroblasts in vitro and skin photo-protection in vivo.
General Physiology and Biophysics. Roč. 32, č. 3 (2013), s. 347-359. ISSN 0231-5882. E-ISSN 1338-4325
Grant ostatní: GA MŠk(CZ) ED1.100/02/0123
Institucionální podpora: RVO:68081707
Klíčová slova: CELL-PROLIFERATION * OXIDATIVE-STRESS * ANTIOXIDANT CAPACITY
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 0.875, rok: 2013

Stress-induced fibroblast senescence is thought to contribute to skin aging. Ultraviolet light (UV) radiation is the most potent environmental risk factor in these processes. An Epilobium angustifolium (EA) extract was evaluated for its capacity to reverse the senescent response of normal human dermal fibroblasts (NHDF) in vitro and to exhibit skin photo-protection in vivo. The HPLC-UV-MS analysis of the EA preparation identified three major polyphenol groups: tannins (oenothein B), phenolic acids (gallic and chlorogenic acids) and flavonoids. EA extract increased the cell viability of senescent NHDF induced by serum deprivation. It diminished connective tissue growth factor and fibronectin gene expressions in senescent NHDF. Down-regulation of the UV-induced release of both matrix metalloproteinase-1 and -3 and the tissue inhibitor of matrix metalloproteinases-1 and -2, and also down-regulation of the gene expression of hyaluronidase 2 were observed in repeatedly UV-irradiated NHDF after EA extract treatment. Interestingly, EA extract diminished the down-regulation of sirtuin 1 dampened by UV-irradiation.
Trvalý link: http://hdl.handle.net/11104/0226793