Hormone profiles in microalgae: Gibberellins and brassinosteroids

0399320 - ÚEB 2014 RIV FR eng J - Článek v odborném periodiku
Stirk, W. A. - Balint, P. - Tarkowská, Danuše - Novák, Ondřej - Strnad, Miroslav - Ördög, V. - van Staden, J.
Hormone profiles in microalgae: Gibberellins and brassinosteroids.
Plant Physiology and Biochemistry. Roč. 70, č. 2013 (2013), s. 348-353. ISSN 0981-9428
Grant ostatní: GA MŠk(CZ) ED0007/01/01
Program: ED
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: Brassinolide * Brassinosteroids * Castasterone
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.352, rok: 2013

Endogenous gibberellins and brassinosteroids were quantified in 24 axenic microalgae strains from the Chlorophyceae, Trebouxiophyceae, Ulvophyceae and Charophyceae microalgae strains after 4 days in culture. This is the first report of endogenous gibberellins being successfully detected in microalgae. Between 18 and 20 gibberellins were quantified in all strains with concentrations ranging from 342.7 pg mg(-1) DW in Raphidocelis subcapitata MACC 317-4746.1 pg mg(-1) DW in Scotiellopsis terrestris MACC 44. Slower growing strains (S. terrestris MACC 44, Gyoerffyana humicola MACC 334, Nautococcus mamillatus MACC 716 and Chlorococcum ellipsoideum MACC 712) exhibited the highest gibberellin contents while lowest levels of gibberellins were found in faster growing strains (R. subcapitata MACC 317 and Coelastrum excentrica MACC 504). In all strains, the active gibberellin detected in the highest concentration was GA(6), the predominant intermediates were GA(15) and GA(53) and the main biosynthetic end products were GAB(13) and GA(51). Gibberellin profiles were similar in all strains except for the presence/absence of GA(12) and GA(12)ald. To date this is the second report of endogenous brassinosteroids in microalgae. Brassinosteroids were detected in all 24 strains with concentrations ranging from 117.3 pg mg(-1) DW in R. subcapitata MACC 317-977.8 pg mg(-1) DW in Klebsormidium flaccidum MACC 692. Two brassinosteroids, brassinolide and castasterone were determined in all the strains. Generally, brassinolide occurred in higher concentrations than castasterone.
Trvalý link: http://hdl.handle.net/11104/0226645