Stabilization of Cytokinin Levels Enhances Arabidopsis Resistance Against Verticillium longisporum

0399219 - ÚEB 2014 RIV US eng J - Článek v odborném periodiku
Reusche, M. - Klásková, J. - Thole, K. - Truskina, J. - Novák, Ondřej - Janz, D. - Strnad, Miroslav - Spíchal, L. - Lipka, V. - Teichmann, T.
Stabilization of Cytokinin Levels Enhances Arabidopsis Resistance Against Verticillium longisporum.
Molecular Plant-Microbe Interactions. Roč. 26, č. 8 (2013), s. 850-860. ISSN 0894-0282. E-ISSN 1943-7706
Grant ostatní: GA MŠk(CZ) ED0007/01/01
Program: ED
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: LEAF SENESCENCE * IPT GENE * BIOCHEMICAL-CHARACTERIZATION
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 4.455, rok: 2013

Verticillium longisporum is a vascular pathogen that infects the Brassicaceae host plants Arabidopsis thaliana and Brassica napus. The soilborne fungus enters the plant via the roots and colonizes the xylem of roots, stems, and leaves. During late stages of infections, Verticillium spp. spread into senescing tissue and switch from biotrophic to a necrotrophic life style. Typical symptoms of V longisporum-induced disease are stunted growth and leaf chlorosis. Expression analyses of the senescence marker genes SENESCENCE-ASSOCIATED GENE12, SENESCENCE-ASSOCIATED GENE13, and WRKY53 revealed that the observed chlorosis is a consequence of premature senescence triggered by Vertkillium infection. Our analyses show that, concomitant with the development of chlorosis, levels of trans-zeatin decrease in infected plants. Potentially, induction of cytokinin oxidase/dehydrogenase expression by Verticillium infection contributes to the observed decreases in cytokinin levels. Stabilization of Arabidopsis cytokinin levels by both pharmacological and genetic approaches inhibits Verticillium proliferation and coincides with reduced disease symptom development. In summary, our results indicate that V longisporum triggers premature plant senescence for efficient host plant colonization.
Trvalý link: http://hdl.handle.net/11104/0226560