Počet záznamů: 1
Transcriptional response of lignin-degrading enzymes to 17 alpha-ethinyloestradiol in two white rots
- 1.0399070 - MBÚ 2014 RIV US eng J - Článek v odborném periodiku
Přenosilová, Lenka jr. - Křesinová, Zdena - Slavíková-Anemori, Anna - Cajthaml, Tomáš - Svobodová, Kateřina
Transcriptional response of lignin-degrading enzymes to 17 alpha-ethinyloestradiol in two white rots.
Microbial Biotechnology. Roč. 6, č. 3 (2013), s. 300-306. ISSN 1751-7907
Grant CEP: GA ČR GAP503/10/0408; GA TA ČR TA01020804
Institucionální podpora: RVO:61388971
Klíčová slova: LACCASE GENE-EXPRESSION * TRAMETES SP AH28-2 * MANGANESE PEROXIDASE
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 3.023, rok: 2013
Fungal, ligninolytic enzymes have attracted a great attention for their bioremediation capabilities. A deficient knowledge of regulation of enzyme production, however, hinders the use of ligninolytic fungi in bioremediation applications. In this work, a transcriptional analyses of laccase and manganese peroxidase (MnP) production by two white rots was combined with determination of pI of the enzymes and the evaluation of 17 alpha-ethinyloestradiol (EE2) degradation to study regulation mechanisms used by fungi during EE2 degradation. In the cultures of Trametes versicolor the addition of EE2 caused an increase in laccase activity with a maximum of 34.2 +/- 6.7 U g(-1) of dry mycelia that was observed after 2 days of cultivation. It corresponded to a 4.9 times higher transcription levels of a laccase-encoding gene (lacB) that were detected in the cultures at the same time. Simultaneously, pI values of the fungal laccases were altered in response to the EE2 treatment. Like T. versicolor, Irpex lacteus was also able to remove 10 mg l(-1) EE2 within 3 days of cultivation. While an increase to I. lacteus MnP activity and MnP gene transcription levels was observed at the later phase of the cultivation. It suggests another metabolic role of MnP but EE2 degradation
Trvalý link: http://hdl.handle.net/11104/0226454
Počet záznamů: 1