Low-cost, easy-to-prepare magnetic chitosan microparticles for enzymes immobilization

0398969 - ÚVGZ 2014 RIV GB eng J - Článek v odborném periodiku
Pospišková, K. - Šafařík, Ivo
Low-cost, easy-to-prepare magnetic chitosan microparticles for enzymes immobilization.
Carbohydrate Polymers. Roč. 96, č. 2 (2013), s. 545-548. ISSN 0144-8617. E-ISSN 1879-1344
Grant CEP: GA MŠMT(CZ) LD13021
Institucionální podpora: RVO:67179843
Klíčová slova: Chitosan * Magnetite * Microwave irradiation * Enzymes immobilization
Kód oboru RIV: CE - Biochemie
Impakt faktor: 3.916, rok: 2013

Extremely simple procedures for the preparation of magnetic chitosan enzyme carriers have been developed and used for the immobilization of lipase and -galactosidase as model enzymes. In the first case commercially available magnetite microparticles were entrapped in chitosan gel, while in the second case magnetic iron oxides microparticles were synthesized in chitosan matrix from ferrous sulfate using microwave irradiation. Immobilized enzymes showed long-term stability without leaching of enzyme from the support and enabled their repeated use without significant loss of activity.
Trvalý link: http://hdl.handle.net/11104/0226426