The added value of single-cell gene expression profiling

0398940 - BTÚ 2014 RIV GB eng J - Článek v odborném periodiku
Stahlberg, A. - Rusňáková, Vendula - Kubista, Mikael
The added value of single-cell gene expression profiling.
Briefings in Functional Genomics. Roč. 12, č. 2 (2013), s. 81-89. ISSN 2041-2649. E-ISSN 2041-2657
Grant CEP: GA MŠMT(CZ) ME10052; GA ČR GAP303/10/1338
Výzkumný záměr: CEZ:AV0Z50520701
Klíčová slova: single-cell biology * RT-qPCR * TUMOR-CELLS
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 3.427, rok: 2013

Cells are the basic unit of life and they have remarkable abilities to respond individually as well as in concert to internal and external stimuli in a specific manner. Studying complex tissues and whole organs requires understanding of cell heterogeneity and responses to stimuli at the single-cell level. In this review, we discuss the potential of single-cell gene expression profiling, focusing on data analysis and biological interpretation. We exemplify several aspects of the added value of single-cell analysis by comparing the same experimental data at both single-cell and cell population level. Data normalization and handling of missing data are two important steps in data analysis that are performed differently at single-cell level compared with cell population level. Furthermore, we discuss how single-cell gene expression data can be viewed and how subpopulations of cells can be identified and characterized.
Trvalý link: http://hdl.handle.net/11104/0226419