Počet záznamů: 1
In Situ Study of Live Specimens in an Environmental Scanning Electron Microscope
- 1.0397947 - ÚPT 2014 RIV US eng J - Článek v odborném periodiku
Tihlaříková, Eva - Neděla, Vilém - Shiojiri, M.
In Situ Study of Live Specimens in an Environmental Scanning Electron Microscope.
Microscopy and Microanalysis. Roč. 19, č. 4 (2013), s. 914-918. ISSN 1431-9276. E-ISSN 1435-8115
Grant CEP: GA ČR GAP102/10/1410
Institucionální podpora: RVO:68081731
Klíčová slova: live biological sample * high-pressure environment * hydration system * in situ observation * Monte Carlo simulation * methodology
Kód oboru RIV: JA - Elektronika a optoelektronika, elektrotechnika
Impakt faktor: 2.161, rok: 2013
In this paper we introduce new methodology for the observation of living biological samples in an environmental scanning electron microscope (ESEM). The methodology is based on an unconventional initiation procedure for ESEM chamber pumping, free from purge-flood cycles, and on the ability to control thermodynamic processes close to the sample. The gradual and gentle change of the working environment from air to water vapor enables the study of not only living samples in dynamic in situ experiments and their manifestation of life (sample walking) but also its experimentally stimulated physiological reactions. Moreover, Monte Carlo simulations of primary electron beam energy losses in a water layer on the sample surface were studied; consequently, the influence of the water thickness on radiation, temperature, or chemical damage of the sample was considered.
Trvalý link: http://hdl.handle.net/11104/0225541
Počet záznamů: 1