Cryopreservation: Technologies, Applications and Risks/Outcomes

0397554 - MBÚ 2014 RIV US eng M - Část monografie knihy
Homolka, Ladislav
Cryopreservation of Filamentous Fungi.
Cryopreservation: Technologies, Applications and Risks/Outcomes. New York: Nova Science Publishers, Inc, 2013 - (Colvert, A.; Coty, H.), s. 1-66. ISBN 978-1-62618-474-9
Grant CEP: GA ČR GAP504/12/0709
Institucionální podpora: RVO:61388971
Klíčová slova: cryopreservation * filamentous fungi
Kód oboru RIV: EE - Mikrobiologie, virologie

Efficient mycological (and generally microbiological) work requires a reliable source of cultures, i.e. well-defined and taxonomically determined starting material, which is ensured by its safe long-term storage. Besides lyophilization, cryopreservation at low temperatures seems to be a very efficient way to attain this goal. Especially the storage in liquid nitrogen has been considered as the best and most widely applicable preservation technique available for fungi, which seems to surpass all others in the ability to preserve genomic and phenotypic features. Different fungal species and even strains can require different cryopreservation protocols. Recently, some new methods of cryopreservation using different carriers of fungal mycelium have been developed and successfully verified on a large number of fungal strains from a broad spectrum of taxonomical groups of fungi. Besides survival, another principal requirement for successful maintenance of fungal strains is the ability to preserve their features unchanged. It is highly desirable to develop new preservation methods or to improve the current ones, combining advantages and eliminating disadvantages of individual techniques. Besides cryopreservation, also other routinely used preservation methods important for the preservation of filamentous fungi are briefly described in this review
Trvalý link: http://hdl.handle.net/11104/0225209