Pretreatment of spent mushroom substrate for enhancing the conversion of fermentable sugar

0397538 - BC 2014 RIV NL eng J - Článek v odborném periodiku
Wu, S. - Lan, Y. - Wu, Z. - Peng, Y. - Chen, S. - Huang, Z. - Xu, L. - Gelbič, Ivan - Guan, X. - Zhang, L. - Zou, S.
Pretreatment of spent mushroom substrate for enhancing the conversion of fermentable sugar.
Bioresource Technology. Roč. 148, NOV 9 (2013), s. 596-600. ISSN 0960-8524. E-ISSN 1873-2976
Grant ostatní: National High Technology Research and Development Program 863(CN) 2011AA10A203; National Natural Science Foundation of China(CN) 31301724; Forestry University(CN) xjq201203; China National Engineering Research Center of Juncao Technology(CN) JCJJ13021
Institucionální podpora: RVO:60077344
Klíčová slova: Bacillus thuringiensis * high performace liquid chromatography * pretreatment
Kód oboru RIV: GC - Pěstování rostlin, osevní postupy
Impakt faktor: 5.039, rok: 2013
http://www.sciencedirect.com/science/article/pii/S0960852413013734#

To develop a cost-effective biopesticide, spent mushroom substrate (SMS) extract was studied as a potential carbon source for cultivating Bacillus thuringiensis (Bt). Several pretreatments were compared to determine the optimal method for degrading cellulose to produce reducing sugars, including dilute sulfuric acid (0.5–2.0% v/v, 50–121 _C, 1 h), sodium hydroxide (0.5–2% w/v, 50–121 _C, 1 h), calcium hydroxide (0.2–4% w/v, 50–121 _C, 1 h), and hot water (50–121 _C, 1 h). Pretreatment was followed by standard enzymatic hydrolysis and fermentation. Results showed that the highest cellulose degradation was obtained using 2% dilute sulfuric acid pretreatment at 121 _C for 1 h, resulting in a high yield of reducing sugar (284.24 g/kg SMS). Sporulation was also highest using the same pretreatment. Use of SMS is not only an alternative way to commercialize Bt-based biopesticide, but also a potential solution for the environmental pollution associated with accumulation of the spent substrate of the mushroom industry.
Trvalý link: http://hdl.handle.net/11104/0228158