Cryptosporidium scrofarum n. sp. (Apicomplexa: Cryptosporidiidae) in domestic pigs (Sus scrofa)

0396845 - BC 2014 RIV NL eng J - Článek v odborném periodiku
Kváč, Martin - Kestřánová, M. - Pinková, Martina - Květoňová, Dana - Kalinová, Jana - Wagnerová, Pavla - Kotková, Michaela - Vitovec, J. - Ditrich, Oleg - McEvoy, J. - Stenger, B. - Sak, Bohumil
Cryptosporidium scrofarum n. sp. (Apicomplexa: Cryptosporidiidae) in domestic pigs (Sus scrofa).
Veterinary Parasitology. Roč. 191, 3-4 (2013), s. 218-227. ISSN 0304-4017. E-ISSN 1873-2550
Grant CEP: GA MŠMT(CZ) LH11061
Institucionální podpora: RVO:60077344 ; RVO:67985904
Klíčová slova: Cryptosporidium scrofarum * Taxonomy * Morphology * Molecular analyses * Transmission studies * Cryptosporidium pig genotype II
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.545, rok: 2013

We describe the morphological, biological, and molecular characteristics of Cryptosporidium pig genotype II and propose the species name Cryptosporidium scrofarum n. sp. to reflect its prevalence in adult pigs worldwide. Oocysts of C. scrofarum are morphologically indistinguishable from C. parvum, measuring 4.81-5.96 mu m (mean = 5.16) x 4.23-5.29 mu m (mean = 4.83) with a length to width ratio of 1.07 +/- 0.06 (n = 400). Oocysts of C scrofarum obtained from a naturally infected pig were infectious for 8-week-old pigs but not 4-week-old pigs. The prepatent period in 8-week-old Cryptosporidium-naive pigs was 4-6 days and the patent period was longer than 30 days. The infection intensity of C scrofarum in pigs was generally low, in the range 250-4000 oocysts per gram of feces. Infected pigs showed no clinical signs of cryptosporidiosis and no pathology was detected. Cryptosporidium scrofarum was not infectious for adult SCID mice, adult BALB/c mice, Mongolian gerbils (Meriones unguiculatus), southern multimammate mice (Mastomys coucha), yellow-necked mice (Apodemus flavicollis), or guinea pigs (Cavia porcellus). Phylogenetic analyses based on small subunit rRNA, actin, and heat shock protein 70 gene sequences revealed that C. scrofarum is genetically distinct from all known Cryptosporidium species.
Trvalý link: http://hdl.handle.net/11104/0224527