Conditionally immortalized stem cell lines from human spinal cord retain regional identity and generate functional V2a interneurons and motorneurons

0394726 - ÚEM 2014 RIV GB eng J - Článek v odborném periodiku
Cocks, G. - Romanyuk, Nataliya - Amemori, Takashi - Jendelová, Pavla - Forostyak, Oksana - Jeffries, A. R. - Perfect, L. - Thuret, S. - Dayanithi, Govindan - Syková, Eva - Price, J.
Conditionally immortalized stem cell lines from human spinal cord retain regional identity and generate functional V2a interneurons and motorneurons.
Stem Cell Research & Therapy. Roč. 4, č. 3 (2013), s. 69. E-ISSN 1757-6512
Grant CEP: GA AV ČR IAA500390902; GA ČR GAP304/11/2373; GA ČR GA13-00939S; GA ČR(CZ) GBP304/12/G069
Výzkumný záměr: CEZ:AV0Z50390703
Institucionální podpora: RVO:68378041
Klíčová slova: neural stem cells * spinal cord * motoneurons
Kód oboru RIV: FH - Neurologie, neurochirurgie, neurovědy
Impakt faktor: 4.634, rok: 2013

Clonal neural stem cell lines were derived from 10-week-old human fetal spinal cord and conditionally immortalized with an inducible form of cMyc. Clonal lines retain a clear transcriptional signature of ventral spinal cord progenitors, differentiate into relevant ventral neuronal subtypes with functional T-, L-, N-, and P/Q-type Ca2+ channels and spontaneous calcium oscillations, and stably engraft into lesioned rat spinal cord without tumorogenicity.
Trvalý link: http://hdl.handle.net/11104/0224301