Ion specific effects of alkali cations on the catalytic activity of HIV-1 protease

0394039 - ÚOCHB 2014 RIV GB eng J - Článek v odborném periodiku
Pokorná, Jana - Heyda, J. - Konvalinka, Jan
Ion specific effects of alkali cations on the catalytic activity of HIV-1 protease.
Faraday Discussions. Roč. 160, č. 1 (2013), s. 359-370. ISSN 1359-6640. E-ISSN 1364-5498
Grant CEP: GA ČR GBP208/12/G016; GA ČR GAP207/11/1798
Institucionální podpora: RVO:61388963
Klíčová slova: HIV-1 protease * ion-protein interaction * Hofmeister series * enzyme kinetics * molecular dynamics
Kód oboru RIV: CE - Biochemie
Impakt faktor: 4.194, rok: 2013

Specific effects of small alkali cations on the HIV protease activity were studied by a combination of experimental and computational techniques. Our molecular dynamic simulations confirm that the affinity of alkali cations to the HIV protease surface follows the Hofmeister series, mostly due to interactions with carboxylate side chain groups of aspartates and glutamates. Accordingly, our experimental data also showed that the initial velocity of peptide substrate hydrolysis in the presence of different alkali cations generally follows the Hofmeister series, with the exception of caesium. The higher catalytic efficiencies (kcat/KM) in the presence of K+ ions in comparison to other alkali cations were observed at corresponding salt concentrations. Furthermore, we observed an unexpected increase in the hydrolysis of a specific substrate at very low salt concentration.
Trvalý link: http://hdl.handle.net/11104/0222419