Počet záznamů: 1
Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling
- 1.0393862 - ÚOCHB 2014 RIV GB eng J - Článek v odborném periodiku
Ménová, Petra - Cahová, Hana - Plucnara, Medard - Havran, Luděk - Fojta, Miroslav - Hocek, Michal
Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling.
Chemical Communications. Roč. 49, č. 41 (2013), s. 4652-4654. ISSN 1359-7345. E-ISSN 1364-548X
Grant CEP: GA ČR GBP206/12/G151
Institucionální podpora: RVO:61388963 ; RVO:68081707
Klíčová slova: cross-coupling reactions * DNA-protein interactions * nucleoside triphosphates * enzymatic incorporation
Kód oboru RIV: CC - Organická chemie
Impakt faktor: 6.718, rok: 2013
Enzymatic construction of single-nucleobase redox-labelled oligo-nucleotides was developed either based on polymerase incorporation of a single modified nucleoside triphosphate (dNTP) followed by primer extension (PEX) with natural dNTPs or based on PEX with a biotinylated one-nucleotide overhang template, magneto-separation and the second PEX with a full-length template.
Trvalý link: http://hdl.handle.net/11104/0222229
Počet záznamů: 1