Specific features of Zn2+, Co2+ and Ni2+ ion binding to DNA in alkaline solutions

0392688 - ÚOCHB 2014 RIV GB eng J - Článek v odborném periodiku
Sorokin, V. A. - Valeev, V. A. - Usenko, E. L. - Rakovsky, Y. P. - Andrushchenko, Valery
Specific features of Zn2+, Co2+ and Ni2+ ion binding to DNA in alkaline solutions.
International Journal of Biological Macromolecules. Roč. 55, Apr (2013), s. 137-141. ISSN 0141-8130. E-ISSN 1879-0003
Grant CEP: GA ČR GAP208/10/0559
Institucionální podpora: RVO:61388963
Klíčová slova: DNA metallization * m-form * alkaline solution * ethidium bromide * DNA-metal complex
Kód oboru RIV: CE - Biochemie
Impakt faktor: 3.096, rok: 2013

Dependence of DNA metallization degree during B-DNA transition into the metallized (m) form on DNA concentration has been studied by visible and differential UV-spectroscopy in the presence of Zn2+, Co2+ and Ni2+ ions in tetraborate buffer (pH 8.5) with and without ethidium bromide. Constants of Mt(2+) binding to double stranded DNA were calculated. The obtained binding constants corresponded to the formation of inter-strand metal bridges stabilizing m-form. Thermodynamic origin of higher efficiency of Zn2+ ions in DNA metallization compared to Co2+ and Ni2+ was revealed. Increase of the DNA helix-coil transition temperature by up to 10 degrees C upon formation of m-form in the presence of Zn2+ ions was observed and rationalized. Furthermore, a strong cooperative decrease (up to 30 degrees C) of the temperature of B m transition induced by heating in the presence of Zn2+ was found and its nature was explained.
Trvalý link: http://hdl.handle.net/11104/0221503