Adhesion and Growth of Vascular Smooth Muscle Cells on Nanostructured and Biofunctionalized Polyethylene

0392341 - FGÚ 2014 RIV CH eng J - Článek v odborném periodiku
Novotná, Katarína - Bačáková, Markéta - Kasálková-Slepičková, N. - Slepička, P. - Lisá, Věra - Švorčík, V. - Bačáková, Lucie
Adhesion and Growth of Vascular Smooth Muscle Cells on Nanostructured and Biofunctionalized Polyethylene.
Materials. Roč. 6, č. 5 (2013), s. 1632-1655. E-ISSN 1996-1944
Grant CEP: GA ČR(CZ) GBP108/12/G108
Institucionální podpora: RVO:67985823
Klíčová slova: plasma treatment * biocompatibility * fibronectin * tissue engineering
Kód oboru RIV: JJ - Ostatní materiály
Impakt faktor: 1.879, rok: 2013

Cell colonization of synthetic polymers can be regulated by physical and chemical modifications of the polymer surface. High-density and low-density polyethylene (HDPE and LDPE) were activated with Ar+ plasma and grafted with fibronectin (Fn) or bovine serum albumin (BSA). Plasma treatment and grafting with Fn or BSA improved the adhesion and growth of vascular smooth muscle cells in a serum-supplement mdium. The final cell ppulation densities on day 6 after seeding were on an average higher on LDPE than on HDPE. In a serum-free medium, BSA grafted to the polymer surface hampered cell adhesion. Thus, the cell behavior on polyethylene can be modulated by its type, intensity of plasma modification, grafting with biomolecules, and composition of the culture medium
Trvalý link: http://hdl.handle.net/11104/0221242