Neuropeptide FF analog RF9 is not an antagonist of NPFF receptor and decreases food intake in mice after its central and peripheral administration

0391980 - ÚOCHB 2014 RIV NL eng J - Článek v odborném periodiku
Maletínská, Lenka - Tichá, Anežka - Nagelová, Veronika - Špolcová, Andrea - Blechová, Miroslava - Elbert, Tomáš - Železná, Blanka
Neuropeptide FF analog RF9 is not an antagonist of NPFF receptor and decreases food intake in mice after its central and peripheral administration.
Brain Research. Roč. 1498, Mar 1 (2013), s. 33-40. ISSN 0006-8993. E-ISSN 1872-6240
Grant CEP: GA ČR GAP303/10/1368
Institucionální podpora: RVO:61388963
Klíčová slova: PrRP * NPFF * RF9 * Binding * MAPK/ERK1/2phosphorylation * food intake
Kód oboru RIV: FH - Neurologie, neurochirurgie, neurovědy
Impakt faktor: 2.828, rok: 2013

Neuropeptide FF (NPFF) belongs to the RF-amide family of peptides bearing the identical C-terminal amino acid sequence (R-F-NH2). In addition to NPFF, prolactin-releasing peptide (PrRP), another RP-amide, binds to NPFF receptors with high affinity A selective antagonist of PrRP has not yet been identified, but a selective antagonist of NPFF, 1-adamantanecarbonyl-RF-NH2 (RF9), was recently reported to antagonize the hyperalgesic effect of NPFF after central administration to mice. In the present study, RF9 competed with NPFF analog D-Y-L-(N-Me)-F-Q-P-Q-R-F-NH2 (1DMe) in binding to CHO-K1 cell membranes transfected with the human NPFF2 receptor. In rat pituitary RC-4B/C cells, where the expression of the NPFF2 receptor was proved by immunodetection, RF9 did not reverse the phosphorylation of MAPK/ERK1/2 induced by PyrINPFF. In vivo experiments with fasted mice confirmed that centrally injected [Tyr(1)]NPFF significantly lowered food intake. However, RF9, a putative NPFF2 antagonist, did not reverse the anorectic effect of [Tyr(1)]NPFF. Paradoxically, RF9 itself exhibited an anorectic effect in fasted mice not only after intracerebroventricular but also after subcutaneous administration. This finding casts doubt on claims that RF9 is an NPFF antagonist.
Trvalý link: http://hdl.handle.net/11104/0220979