Comparative study on low resolution structures of apoferritin via SANS and SAXS

0391899 - ÚMCH 2014 RIV GB eng J - Článek v odborném periodiku
Kuklin, A. I. - Murugova, T. N. - Ivankov, O. I. - Rogachev, A. V. - Soloviov, D. V. - Kovalev, Yu. S. - Ishchenko, A. V. - Zhigunov, Alexander - Kurkin, T. S. - Gordeliy, V. I.
Comparative study on low resolution structures of apoferritin via SANS and SAXS.
Journal of Physics: Conference Series. Roč. 351, č. 1 (2012), 012009_1-012009_9. ISSN 1742-6588. E-ISSN 1742-6596.
[SANS-YuMO User Meeting. Dubna, 27.05.2011-30.05.2011]
Výzkumný záměr: CEZ:AV0Z40500505
Klíčová slova: apoferritin * SANS * SAXS
Kód oboru RIV: CD - Makromolekulární chemie

The results of small angle scattering investigation of protein apoferritin are presented. The sizes and shapes, including those determined by indirect Fourier transform method, are calculated. The pair-distance distribution function for both small angle neutron (SANS) and X-ray small angle scattering (SAXS) are obtained. It is shown that SANS and SAXS methods give similar shape (spherical shell with holes) of apoferritin. At the same time, fits of experimental data for SAXS and SANS curves give a little different sizes and volumes of the molecule. The reasons for these differences are discussed.
Trvalý link: http://hdl.handle.net/11104/0222039