Neonatal colonization by Bifidobacterium longum prevents systemic sensitization to Bet v 1 in mouse gnotobiotic model of birch pollen allergy

0390683 - MBÚ 2013 CZ eng A - Abstrakt
Schwarzer, Martin - Šrůtková, Dagmar - Schabussova, I. - Hudcovic, Tomáš - Wiedermann, U. - Kozáková, Hana
Neonatal colonization by Bifidobacterium longum prevents systemic sensitization to Bet v 1 in mouse gnotobiotic model of birch pollen allergy.
Sympozium Společnosti pro probiotika a prebiotika /7./. Praha: Česká zemědělská univerzita Praha, eds. J. Nevoral a V. Rada, 2012. s. 12-12. ISBN 978-80-213-2271-4.
[Sympozium Společnosti pro probiotika a prebiotika /7./. 28.03.2012, Praha]
Institucionální podpora: RVO:61388971
Klíčová slova: Germ-free mice * Bifidobacterium * probiotics
Kód oboru RIV: EC - Imunologie

Wild-type and TLR2-/-, TLR4-/- and MyD88-/- deficient mouse bone marrow-derived dendritic cells were stimulated with formalin inactivated B. longum. BALB/c mice were monocolonized by bifidobacteria immediately after birth naturally from their gnotobiotic mothers in plastic isolator. Two-month old monocolonized mice and age-matched germ-free controls were three times subcutaneously immunized with major birch pollen allergen Bet v 1/Alum in two-week interval. Mice were sacrificed one week after the last immunization. Stimulation of murine dendritic cells by B. longum induced only low levels of co-stimulatory molecules CD40, CD80 and CD86 accompanied by production of IL-10, which was TLR2- and MyD88-depend. Bifidobacterium-colonized mice showed a significantly reduced level of allergen-specific antibody IgE, IgG2a, IgG1 and total IgE in blood sera compared to germ-free controls
Trvalý link: http://hdl.handle.net/11104/0219553