Transgenic RNAi in mouse oocytes: The first decade

0390084 - ÚMG 2013 RIV NL eng J - Článek v odborném periodiku
Malík, Radek - Svoboda, Petr
Transgenic RNAi in mouse oocytes: The first decade.
Animal Reproduction Science. Roč. 134, 1-2 (2012), s. 64-68. ISSN 0378-4320. E-ISSN 1873-2232
Výzkumný záměr: CEZ:AV0Z50520514
Institucionální podpora: RVO:68378050
Klíčová slova: RNAi * oocyte * transgene * silencing
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 1.897, rok: 2012

RNA interference (RNAi), a sequence-specific mRNA degradation induced by double-stranded RNA (dsRNA), is a common approach employed to specifically silence genes. Experimental RNAi in plant and invertebrate models is frequently induced by long dsRNA. However, in mammals, short RNA molecules are used preferentially since long dsRNA can provoke sequence-independent type I interferon response. A notable exception are mammalian oocytes where the interferon response is suppressed and long dsRNA is a potent and specific trigger of RNAi. Transgenic RNAi is an adaptation of RNAi allowing for inducing sequence-specific silencing upon expression of dsRNA. A decade ago, we have developed a vector for oocyte-specific expression of dsRNA, which has been used to study gene function in mouse oocytes on numerous occasions. This review provides an overview and discusses benefits and drawbacks encountered by us and our colleagues while working with the oocytes-specific transgenic RNAi system.
Trvalý link: http://hdl.handle.net/11104/0219036