Pseudoscardovia suis gen. nov., sp. nov., a new member of the family Bifidobacteriaceae isolated from the digestive tract of wild pigs (Sus scrofa)

0389653 - ÚŽFG 2013 RIV DE eng J - Článek v odborném periodiku
Killer, Jiří - Mrázek, Jakub - Bunešová, V. - Havlík, J. - Koppová, Ingrid - Benada, Oldřich - Rada, V. - Kopečný, Jan - Vlková, E.
Pseudoscardovia suis gen. nov., sp. nov., a new member of the family Bifidobacteriaceae isolated from the digestive tract of wild pigs (Sus scrofa).
Systematic and Applied Microbiology. Roč. 36, č. 1 (2013), 11-16. ISSN 0723-2020. E-ISSN 1618-0984
Grant CEP: GA ČR GA523/08/1091
Grant ostatní: GA MZe(CZ) QJ1210093
Program: QJ
Institucionální podpora: RVO:67985904 ; RVO:61388971
Klíčová slova: Bifidobacteriaceae * Digestive tract * Multilocus sequence analysis
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 3.310, rok: 2013

Seventeen fructose-6-phosphate phosphoketolase-positive bacterial strains were isolated from the digestive tract of wild pigs (Sus scrofa). Most of them were identified as Bifidobacterium boum according to sequences of 16S rRNA gene. Two strains isolated from the small intestine content had unusual morphology of cells in comparison with bifidobacteria. Cells growing in liquid anaerobic media were regular shaped rods arranged mostly in pairs. These isolates showed relatively low 16S rRNA gene sequence similarities (maximum identity of 94%) to members of the family Bifidobacteriaceae. Nevertheless, phylogenetic analyses of 16S rRNA, hsp60 and xfp gene sequences revealed that these strains are more related to recently described Neoscardovia, Aeriscardovia and other scardovial genera, than to Bifidobacterium species. Partial gene sequences of other phylogenetic markers showed low (65.8-89.5%) similarities to genome sequences of bifidobacteria and Gardnerella vaginalis. The major fatty acids detected in cells of the representative strain DPTE4 T were C 16:0, C 18:1, C 14:0. The peptidoglycan type of the DPTE4 T strain was A3βl-Orn(l-Lys)-l-Ser(l-Ala)-l-Ala 2. Polar lipid analysis revealed two phosphoglycolipids and phospholipids, a glycolipid and diphosphatidylglycerol. The results of phylogenetic, genotypic and phenotypic analyses support the proposal of a novel taxa, Pseudoscardovia suis gen. nov., sp. nov. (type strain = DPTE4 T = DSM 24744 T = CCM 7942 T).
Trvalý link: http://hdl.handle.net/11104/0218538