Thermodynamic stability and energetics of DNA duplexes containing major intrastrand cross-links of second-generation antitumor dinuclear Pt-II complexes

0389601 - BFÚ 2013 RIV US eng J - Článek v odborném periodiku
Florian, Jakub - Kašpárková, Jana - Farell, N.P. - Brabec, Viktor
Thermodynamic stability and energetics of DNA duplexes containing major intrastrand cross-links of second-generation antitumor dinuclear Pt-II complexes.
Journal of Biological Inorganic Chemistry. Roč. 17, č. 2 (2012), s. 187-196. ISSN 0949-8257. E-ISSN 1432-1327
Grant CEP: GA ČR(CZ) GD301/09/H004; GA ČR(CZ) GAP301/10/0598; GA MŠMT(CZ) ME10066
Institucionální podpora: RVO:68081707
Klíčová slova: NUCLEOTIDE EXCISION-REPAIR * PLATINUM ANTICANCER DRUGS * GROUP DOMAIN PROTEINS
Kód oboru RIV: BO - Biofyzika
Impakt faktor: 3.353, rok: 2012

The effects of major DNA intrastrand cross-links of antitumor dinuclear Pt-II complexes [{trans-PtCl(NH3)(2)}(2)-mu-{trans-(H2N(CH2)(6)NH2(CH2)(2)NH2(CH2)(6) NH2)}](4+) (1) and [{PtCl(DACH)}(2)-mu-{H2N(CH2)(6)NH2 (CH2)(2)NH2(CH2)(6)NH2)}](4+) (2) (DACH is 1,2-diaminocyclohexane) on DNA stability were studied with emphasis on thermodynamic origins of that stability. Oligodeoxyribonucleotide duplexes containing the single 1,2, 1,3, or 1,5 intrastrand cross-links at guanine residues in the central TGGT, TGTGT, or TGTTTGT sequences, respectively, were prepared and analyzed by differential scanning calorimetry. The unfolding of the platinated duplexes was accompanied by unfavorable free energy terms.
Trvalý link: http://hdl.handle.net/11104/0218466