Chemical Cross-Linking and H/D Exchange for Fast Refinement of Protein Crystal Structure

0389121 - MBÚ 2013 RIV US eng J - Článek v odborném periodiku
Rozbeský, Daniel - Man, Petr - Kavan, Daniel - Chmelík, Josef - Černý, Jiří - Bezouška, Karel - Novák, Petr
Chemical Cross-Linking and H/D Exchange for Fast Refinement of Protein Crystal Structure.
Analytical Chemistry. Roč. 84, č. 2 (2012), s. 867-870. ISSN 0003-2700. E-ISSN 1520-6882
Grant CEP: GA ČR(CZ) GAP207/10/1040; GA ČR GA303/09/0477; GA ČR GD305/09/H008; GA MŠMT LC545
Výzkumný záměr: CEZ:AV0Z50520701; CEZ:AV0Z50200510
Klíčová slova: MASS-SPECTROMETRY * chemical cross-linking * NKR-P1A receptor
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 5.695, rok: 2012

A combination of chemical cross-linking and hydrogen−deuterium exchange coupled to high resolution mass spectrometry was used to describe structural differences of NKR-P1A receptor. The loop region extended from the compact core in the crystal structure was found to be closely attached to the protein core in solution. Our approach has potential to refine protein structures in solution within a few days and has very low sample consumption
Trvalý link: http://hdl.handle.net/11104/0218019