Long-Term Acclimation of the Cyanobacterium Synechocystis sp PCC 6803 to High Light Is Accompanied by an Enhanced Production of Chlorophyll That Is Preferentially Channeled to Trimeric Photosystem I

0388823 - MBÚ 2013 RIV US eng J - Článek v odborném periodiku
Kopečná, Jana - Komenda, Josef - Bučinská, Lenka - Sobotka, Roman
Long-Term Acclimation of the Cyanobacterium Synechocystis sp PCC 6803 to High Light Is Accompanied by an Enhanced Production of Chlorophyll That Is Preferentially Channeled to Trimeric Photosystem I.
Plant Physiology. Roč. 160, č. 4 (2012), s. 2239-2250. ISSN 0032-0889. E-ISSN 1532-2548
Grant CEP: GA MŠMT(CZ) ED2.1.00/03.0110; GA ČR GAP501/10/1000; GA ČR GBP501/12/G055
Institucionální podpora: RVO:61388971
Klíčová slova: CAB-LIKE-PROTEINS * SP PCC-6803 * PHOTOSYNTHETIC APPARATUS
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 6.555, rok: 2012

Cyanobacteria acclimate to high-light conditions by adjusting photosystem stoichiometry through a decrease of photosystem I (PSI) abundance in thylakoid membranes. As PSI complexes bind the majority of chlorophyll (Chl) in cyanobacterial cells, it is accepted that the mechanism controlling PSI level/synthesis is tightly associated with the Chl biosynthetic pathway. However, how Chl is distributed to photosystems under different light conditions remains unknown. Using radioactive labeling by S-35 and by C-14 combined with native/two-dimensional electrophoresis, we assessed the synthesis and accumulation of photosynthetic complexes in parallel with the synthesis of Chl in Synechocystis sp. PCC 6803 cells acclimated to different light intensities. Although cells acclimated to higher irradiances (150 and 300 mu E m(-2)s(-1)) exhibited markedly reduced PSI content when compared with cells grown at lower irradiances (10 and 40 mu E m(-2)s(-1)), they grew much faster and synthesized significantly more Chl, as well as both photosystems
Trvalý link: http://hdl.handle.net/11104/0217729