Electrophoretic characterization of seminal plasma and sperm proteins in patient with normal and pathological spermiograms

0388692 - BTÚ 2013 CZ eng A - Abstrakt
Postlerová, Pavla - Jonáková, Věra - Pěknicová, Jana
Electrophoretic characterization of seminal plasma and sperm proteins in patient with normal and pathological spermiograms.
Book of abstract. Praha: Biotechnologický ústav v. v. i. AVČR, 2010 - (Pěknicová, J.). s. 49-49
[XVI. symposium českých reprodukčních imunologů s mezinárodní účastí. 28.05.2010-30.05.2010, Žďár nad Sázavou]
Grant CEP: GA MŠMT(CZ) 1M06011; GA ČR(CZ) GA523/08/H064; GA ČR(CZ) GA303/09/1285; GA MZd NS10009-4/2008
Výzkumný záměr: CEZ:AV0Z50520701
Klíčová slova: Human sperm * Seminal plasma protein * Hyaluronidase * Proteolytic activity
Kód oboru RIV: CE - Biochemie

We characterized proteins of seminal plasma and spermatozoa of patient with different spermiograms by electrophoretic methods. Protein profiles of sperm from men with normal and pathological spermiograms were compared. Futher characterization was carried out by detection of glycoproteins and proteolytic activity. Quantitative and qualitative changes in proteins and decreased or increased activity of some enzymes can negative impact sperm fertilizating ability. Protein identification or determination of enzymatic activity in ejaculates might help explain the background of fertilizing failure in men with pathological spermiograms and provide tools its detection.
Trvalý link: http://hdl.handle.net/11104/0218092