Production of high-density Chlorella culture grown in fermenters

0388567 - MBÚ 2013 RIV US eng J - Článek v odborném periodiku
Doucha, Jiří - Lívanský, Karel
Production of high-density Chlorella culture grown in fermenters.
Journal of Applied Phycology. Roč. 24, č. 1 (2012), s. 35-43. ISSN 0921-8971. E-ISSN 1573-5176
Grant CEP: GA MŠMT OE09025
Institucionální podpora: RVO:61388971
Klíčová slova: Chlorella vulgaris * Heterotrophic culture * Fed-batch
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.326, rok: 2012

The freshwater microalga Chlorella vulgaris was grown heterotrophically in fed-batch 50-600-L fermenters at 36A degrees C, on aerated and mixed nutrient solution with urea as a nitrogen and glucose as a carbon and energy source. Cell density increased from the initial value 6.25 to 117.18 g DW L-1 in 32 h in the fermenter 50 L at a mean growth rate 3.52 g DW L-1 h(-1). The DW increase in the fermenter 200 L was from 7.25 to 94.82 g DW L-1 in 26.5 h at a mean growth rate 3.37 g DW L-1 h(-1). Mean specific growth rate mu was about 0.1 h(-1) in the both fermenters, if nutrients and oxygen were adequately supplied. The DW increase in the fermenter 600 L was from 0.8 to 81.6 g DW L-1 in 66.5 h at a mean growth rate 1.22 g DW L-1 h(-1) and mu = 0.07 h(-1). A limitation of the cell growth rate in 600 L fermenter caused by a low dissolved oxygen concentration above cell densities higher than 10 g DW L-1) occurred. Specific growth rate decreased approximately linearly with increasing glucose concentration (25-80 g glucose L-1) at the beginning of cultivation and decreased with the time of cultivation. The cell yield was 0.55-0.69 g DW (g glucose)(-1). The content of proteins, beta-carotene, and chlorophylls in the cells steadily increased and starch content decreased, by keeping aerated and mixed culture another 12 h in fermenter after the cell growth was stopped due to glucose deficiency
Trvalý link: http://hdl.handle.net/11104/0217464