An unexpected gene cluster for downstream degradation of alkylphenols in Sphingomonas sp strain TTNP3

0387875 - ÚMG 2013 RIV DE eng J - Článek v odborném periodiku
Kolvenbach, B.A. - Dobrowinski, H. - Fousek, Jan - Vlček, Čestmír - Schaffer, A. - Gabriel, F.L.P. - Kohler, H.P.E. - Corvini, P.F.X.
An unexpected gene cluster for downstream degradation of alkylphenols in Sphingomonas sp strain TTNP3.
Applied Microbiology and Biotechnology. Roč. 93, č. 3 (2012), s. 1315-1324. ISSN 0175-7598. E-ISSN 1432-0614
Grant CEP: GA MŠMT(CZ) 1M0520
Institucionální podpora: RVO:68378050
Klíčová slova: hydroquinone * degradation * Sphingomonas * nonylphenol * bisphenol A
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 3.689, rok: 2012

In silico analysis of nucleotide sequences flanking the recently found hydroquinone dioxygenase in Sphingomonas sp. strain TTNP3 revealed a gene cluster that encodes a hydroquinone catabolic pathway. In addition to the two open-reading frames encoding the recently characterized hydroquinone dioxygenase, the cluster consisted of six open-reading frames. We were able to express the three open-reading frames, hqdC, hqdD, and hqdE, and demonstrated that the three gene products, HqdC, HqdD, and HqdE had 4-hydroxymuconic semialdehyde dehydrogenase, maleylacetate reductase, and intradiol dioxygenase activity, respectively. Surprisingly, the gene cluster showed similarities to functionally related clusters found in members of the alpha- and gamma-proteobacteria rather than to those found in other members of the genus Sphingomonas sensu latu.
Trvalý link: http://hdl.handle.net/11104/0218431