Plant Salt Tolerance: Methods and Protocols

0387792 - ÚEB 2013 RIV US eng M - Část monografie knihy
Dobrev, Petre - Vaňková, Radomíra
Quantification of Abscisic Acid, Cytokinin, and Auxin Content in Salt-Stressed Plant Tissues.
Plant Salt Tolerance: Methods and Protocols. Vol. 913. New York: Humana Press, 2012 - (Shabala, S.; Cuin, T.), s. 251-261. Methods in Molecular Biology. ISBN 978-1-61779-985-3
Grant CEP: GA MŠMT(CZ) LD11073
Výzkumný záměr: CEZ:AV0Z50380511
Klíčová slova: Abscisic acid * Auxin * Cytokinin
Kód oboru RIV: ED - Fyziologie
http://home.ueb.cas.cz/publikace/2012_Dobrev.pdf

Plant hormones cytokinins, auxin (indole-3-acetic acid), and abscisic acid are central to regulation of plant growth and defence to abiotic stresses such as salinity. Quantification of the hormone levels and determination of their ratios can reveal different plant strategies to cope with the stress, e.g., suppression of growth or mobilization of plant metabolism. This chapter describes a procedure enabling such quantification. Due to the high variability of these hormones in plant tissues, it is advantageous to determine their content in the same sample. Reverse phase and ion exchange chromatography allows separation of the individual hormone fractions. Hormones as well as their metabolites can be identified and quantified by LC/MS.
Trvalý link: http://hdl.handle.net/11104/0220336