Biotransformation of benzonitrile herbicides via the nitrile hydratase-amidase pathway in rhodococci

0387021 - MBÚ 2013 RIV DE eng J - Článek v odborném periodiku
Veselá, Alicja Barbara - Pelantová, Helena - Šulc, Miroslav - Macková, M. - Lovecká, P. - Thimová, P. - Pasquarelli, F. - Pičmanová, Martina - Pátek, Miroslav - Bhalla, T. C. - Martínková, Ludmila
Biotransformation of benzonitrile herbicides via the nitrile hydratase-amidase pathway in rhodococci.
Journal of Industrial Microbiology & Biotechnology. Roč. 39, č. 12 (2012), s. 1811-1819. ISSN 1367-5435. E-ISSN 1476-5535
Grant CEP: GA MŠMT OC09046; GA ČR(CZ) GAP504/11/0394; GA ČR GD305/09/H008
Klíčová slova: Nitrile hydratase * Amidase * Benzonitrile herbicides
Kód oboru RIV: EE - Mikrobiologie, virologie
Impakt faktor: 2.321, rok: 2012

The aim of this work was to determine the ability of rhodococci to transform 3,5-dichloro-4-hydroxybenzonitrile (chloroxynil), 3,5-dibromo-4-hydroxybenzonitrile (bromoxynil), 3,5-diiodo-4-hydroxybenzonitrile (ioxynil) and 2,6-dichlorobenzonitrile (dichlobenil); to identify the products and determine their acute toxicities. Rhodococcus erythropolis A4 and Rhodococcus rhodochrous PA-34 converted benzonitrile herbicides into amides, but only the former strain was able to hydrolyze 2,6-dichlorobenzamide into 2,6-dichlorobenzoic acid, and produced also more of the carboxylic acids from the other herbicides compared to strain PA-34. Transformation of nitriles into amides decreased acute toxicities for chloroxynil and dichlobenil, but increased them for bromoxynil and ioxynil. The amides inhibited root growth in Lactuca sativa less than the nitriles but more than the acids. The conversion of the nitrile group may be the first step in the mineralization of benzonitrile herbicides but cannot be itself considered to be a detoxification
Trvalý link: http://hdl.handle.net/11104/0217221