Organ growth without cell division: somatic polyploidy in a moth, Ephestia kuehniella

0384781 - BC 2013 RIV CA eng J - Článek v odborném periodiku
Buntrock, L. - Marec, František - Krueger, S. - Traut, W.
Organ growth without cell division: somatic polyploidy in a moth, Ephestia kuehniella.
Genome. Roč. 55, č. 11 (2012), s. 755-763. ISSN 0831-2796. E-ISSN 1480-3321
Grant CEP: GA AV ČR IAA600960925
Institucionální podpora: RVO:60077344
Klíčová slova: genome size * C-value * cell size
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 1.668, rok: 2012

Organ growth depends on cell division and cell growth. Here, we present a study on two organs whose growth depends entirely on cell growth, Malpighian tubules and silk glands of Ephestia kuehniella. We determined the number of endocyles required to reach these stages by Feulgen cytometry. Cells of Malpighian tubules were in the 2C stage in first instar larvae and reached 1024C after 9 endocycles in last instar larvae (1C = 0.45 pg DNA). Silk gland cells already reached a DNA content of 8C-16C in first instar larvae and attained up to 8192C in last instar larvae after a total of 12 endocycles. The nuclei were small and spherical in first instar larvae, but they were huge, flat, and bizarrely branched in last instar larvae. We consider branching as a compensatory adaptation to improve molecular traffic between nucleus and cytoplasm in highly polyploid cells (i) by reducing the mean distance between nucleus and cytoplasm and (ii) by enlarging the surface-to-volume ratio of these nuclei.
Trvalý link: http://hdl.handle.net/11104/0215794