Molecular differentiation of three loach species (Pisces, Cobitidae) based on the nuclear 5S rDNA marker

0384520 - ÚBO 2013 RIV PL eng J - Článek v odborném periodiku
Kirtiklis, L. - Boron, A. - Ptasznik, P. - Lusková, Věra - Lusk, Stanislav
Molecular differentiation of three loach species (Pisces, Cobitidae) based on the nuclear 5S rDNA marker.
Folia Biologica-Krakow. Roč. 59, 3-4 (2011), s. 141-145. ISSN 0015-5497. E-ISSN 1734-9168
Výzkumný záměr: CEZ:AV0Z60930519
Klíčová slova: Cobitis * PCR-RFLP * Sabanejewia * species identification * 5S rDNA
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 0.657, rok: 2011

The diversity of the 5S rDNA fragment among three loach species: Cobitis taenia, C. elongatoides and Sabanejewia aurata was investigated using universal PCR primers for this gene. Three amplification products were obtained: 220 bp length for C. taenia and C. elongatoides, and 330 bp for S. aurata. Two amplicons with the same length (in Cobitis) were digested with TaqI restriction endonuclease. This enzyme found one restriction site T/CGA in the C. elongatoides fragment, while in the case of C. taenia no cleavage effect was observed. On this basis we constructed an easy and cheap method for loach species discrimination. It seems adequate for effective support of conservation initiatives for endangered loathes.
Trvalý link: http://hdl.handle.net/11104/0214143