Nuclear LSm8 affects number of cytoplasmic processing bodies via controlling cellular distribution of Like-Sm proteins

0381677 - ÚMG 2013 RIV US eng J - Článek v odborném periodiku
Novotný, Ivan - Podolská, Kateřina - Blažíková, Michaela - Valášek, Leoš Shivaya - Svoboda, Petr - Staněk, David
Nuclear LSm8 affects number of cytoplasmic processing bodies via controlling cellular distribution of Like-Sm proteins.
Molecular Biology of the Cell. Roč. 23, č. 19 (2012), s. 3776-3785. ISSN 1059-1524. E-ISSN 1939-4586
Grant CEP: GA AV ČR KAN200520801; GA ČR GA204/07/0133; GA ČR GAP305/10/2215; GA ČR GAP302/11/1910; GA ČR(CZ) GBP305/12/G034
Výzkumný záměr: CEZ:AV0Z50390703; CEZ:AV0Z50520514; CEZ:AV0Z50200510
Institucionální podpora: RVO:68378050 ; RVO:68378041 ; RVO:61388971
Klíčová slova: P-bodies * LSm proteins * mRNA degradation
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 4.803, rok: 2012

Processing bodies (P-bodies) are dynamic cytoplasmic structures involved in mRNA degradation, but the mechanism that governs their formation is poorly understood. In this paper, we address a role of Like-Sm (LSm) proteins in formation of P-bodies and provide evidence that depletion of nuclear LSm8 increases the number of P-bodies, while LSm8 overexpression leads to P-body loss. We show that LSm8 knockdown causes relocalization of LSm4 and LSm6 proteins to the cytoplasm and suggest that LSm8 controls nuclear accumulation of all LSm2-7 proteins. We propose a model in which redistribution of LSm2-7 to the cytoplasm creates new binding sites for other P-body components and nucleates new, microscopically visible structures. The model is supported by prolonged residence of two P-body proteins, DDX6 and Ago2, in P-bodies after LSm8 depletion, which indicates stronger interactions between these proteins and P-bodies. Finally, an increased number of P-bodies has negligible effects on microRNA-mediated translation repression and nonsense mediated decay, further supporting the view that the function of proteins localized in P-bodies is independent of visible P-bodies.
Trvalý link: http://hdl.handle.net/11104/0216400