Biological and molecular analysis of the pathogenic variant C3 of potato spindle tuber viroid (PSTVd) evolved during adaptation to chamomile (Matricaria chamomilla)

0381545 - BC 2013 RIV DE eng J - Článek v odborném periodiku
Matoušek, Jaroslav - Stehlík, Jan - Procházková, Jitka - Orctová, Lidmila - Wullenweber, J. - Füssy, Zoltán - Kováčik, J. - Duraisamy, Ganesh Selvaraj - Ziegler, A. - Schubert, J. - Steger, G.
Biological and molecular analysis of the pathogenic variant C3 of potato spindle tuber viroid (PSTVd) evolved during adaptation to chamomile (Matricaria chamomilla).
Biological Chemistry. Roč. 393, č. 7 (2012), s. 605-615. ISSN 1431-6730. E-ISSN 1437-4315
Grant CEP: GA ČR GCP501/10/J018
Výzkumný záměr: CEZ:AV0Z50510513
Institucionální podpora: RVO:60077344
Klíčová slova: viroid-specific small RNA profiles * micro RNA probes * viroid adaptation * biolistic inoculation of plants * viroid pathogenesis markers
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.683, rok: 2012

Viroid-caused pathogenesis is a specific process dependent on viroid and host genotype(s), and may involve viroid-specific small RNAs (vsRNA). We describe a new PSTVd variant C3, evolved through sequence adaptation to the host chamomile, causing extraordinary severe symptoms. The symptoms and the vsRNA pool of PSTVd-C3 were compared to those of severe variant PSTVd-AS1, from which PSTVd-C3 differs by 5 mutations within the P domain. PSTVd-C3 and -AS1 caused similar symptoms on chamomile, tomato, and Nicotiana benthamiana, including a block of lignin biosynthesis in roots of cultured chamomile and tomato. These strains exhibited similar but species-specific distributions of selected vsRNAs, as quantified using TaqMan probes, with higher stability and lower variation in analyzed vsRNA pools than the mild PSTVd-QFA. Strong downregulation of four selected ‘expression markers’ (TCP3, CIPK, VSF-1, and VPE) was detected in tomato leaf blades infected by PSTVd-C3- and -AS1 but not by PSTVd-QFA.
Trvalý link: http://hdl.handle.net/11104/0211990