First Report of Hop stunt viroid Infecting Hop in Slovenia

0381533 - BC 2013 RIV US eng O - Ostatní výsledky
Radisek, S. - Majer, S. - Jakse, S. - Javornik, B. - Matoušek, Jaroslav
First Report of Hop stunt viroid Infecting Hop in Slovenia.
2012
Grant CEP: GA ČR GCP501/10/J018
Výzkumný záměr: CEZ:AV0Z50510513
Institucionální podpora: RVO:60077344
Klíčová slova: Hop stunt viroid * reverse transcription-PCR detection * pospiviroid
Kód oboru RIV: EE - Mikrobiologie, virologie

Since 2007, symptoms similar to Hop stunt viroid (HSVd) infection have been observed in several hop gardens in Slovenia. Symptoms include stunting, leaf curl, small cones, and dry root rot. In the first year, the incidence varied from 1 to 30% and increased rapidly (by as much as 10%) each subsequent year, mostly along plant rows. For molecular identification of the pathogen, RNA was extracted from leaves and cones of symptomatic and asymptomatic plants from two different hop gardens. Reverse transcription-PCR was carried out using two pairs of specific HSVd primers. Both primer pairs gave a specific PCR product from symptomatic plant tissue, but not from asymptomatic samples. PCR products were subjected to sequencing. Five sequences obtained had 96-99% sequence identity with various HSVd variants from GenBank. To our knowledge, this is the first report of HSVd on hop in Europe. Strict phytosanitary measures have been taken to prevent further spread and to eradicate HSVd infections.
Trvalý link: http://hdl.handle.net/11104/0211980