Uptake and incorporation of sialic acid by the tick Ixodes ricinus

0379767 - BC 2013 RIV GB eng J - Článek v odborném periodiku
Vancová, Marie - Štěrba, J. - Dupejová, Jarmila - Simonová, Z. - Nebesářová, Jana - Novotný, M. V. - Grubhoffer, Libor
Uptake and incorporation of sialic acid by the tick Ixodes ricinus.
Journal of Insect Physiology. Roč. 58, č. 9 (2012), s. 1277-1287. ISSN 0022-1910. E-ISSN 1879-1611
Grant CEP: GA AV ČR KJB600960906; GA ČR GA206/09/1782; GA ČR GD206/09/H026; GA MŠMT(CZ) LC06009
Výzkumný záměr: CEZ:AV0Z60220518
Klíčová slova: Tick * Ixodes ricinus * Sialic acid * Basement membrane * Mass spectrometry * Electron microscopy
Kód oboru RIV: EB - Genetika a molekulární biologie
Impakt faktor: 2.379, rok: 2012

We describe the detection of sialylated N-linked glycans in partially fed Ixodes ricinus tick females using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry. Sialylated glycans were detected in salivary glands as well as in tick guts and we propose the host origin of these structures. In addition, we mapped the transport of sialylated structures from the blood meal through the gut to the salivary glands using electron microscopy. Specific localization of sialylated glycans to basement membranes of salivary glands was observed. Finally, the influence of the sample preparation methods for electron microscopy on ultrastructure and immunogold labeling was evaluated.
Trvalý link: http://hdl.handle.net/11104/0210649