Nanofibers prepared by needleless electrospinning technology as scaffolds for wound healing

0379338 - ÚEM 2013 RIV GB eng J - Článek v odborném periodiku
Dubský, M. - Kubinová, Šárka - Širc, Jakub - Voska, L. - Zajíček, R. - Zajícová, Alena - Lesný, Petr - Jirkovská, A. - Michálek, Jiří - Munzarová, M. - Holáň, Vladimír - Syková, Eva
Nanofibers prepared by needleless electrospinning technology as scaffolds for wound healing.
Journal of Materials Science-Materials in Medicine. Roč. 23, č. 4 (2012), s. 931-941. ISSN 0957-4530. E-ISSN 1573-4838
Grant CEP: GA ČR(CZ) GA304/07/1129; GA AV ČR(CZ) KAN200520804; GA AV ČR(CZ) IAA500390902; GA MŠMT(CZ) 2B06130; GA MŠMT(CZ) 1M0538; GA MŠMT 1M0506
Grant ostatní: GA MZd(CZ) MZO00023001
Výzkumný záměr: CEZ:AV0Z50390703; CEZ:AV0Z40500505; CEZ:AV0Z50520514
Klíčová slova: nanofibers * electrospinning * wound healing
Kód oboru RIV: FH - Neurologie, neurochirurgie, neurovědy
Impakt faktor: 2.141, rok: 2012

Electrospun gelatin and poly-epsilon-caprolactone (PCL) nanofibers were prepared using needleless technology and their biocompatibility and therapeutic efficacy have been characterized in vitro in cell cultures and in an experimental model of a skin wound. The effect of nanofibers on wound healing was examined using a full thickness wound model in rats and compared with a standard control treatment with gauze. Significantly faster wound closure was found with gelatin after 5 and 10 days of treatment, but no enhancement with PCL nanofibers was observed. Histological analysis revealed enhanced epithelialisation, increased depth of granulation tissue and increased density of myofibroblasts in the wound area with gelatin nanofibers. The results show that gelatin nanofibers produced by needleless technology accelerate wound healing and may be suitable as a scaffold for cell transfer and skin regeneration.
Trvalý link: http://hdl.handle.net/11104/0210576