The impact of long-term CO2 enrichment on sun and shade needles of Norway spruce (Picea abies): Photosynthetic performance, needle anatomy and phenolics accumulation

0379249 - ÚVGZ 2013 RIV IE eng J - Článek v odborném periodiku
Lhotáková, Z. - Urban, Otmar - Dubánková, M. - Cvikrová, Milena - Tomášková, Ivana - Kubínová, Lucie - Zvára, K. - Marek, Michal V. - Albrechtová, J.
The impact of long-term CO2 enrichment on sun and shade needles of Norway spruce (Picea abies): Photosynthetic performance, needle anatomy and phenolics accumulation.
Plant Science. 188-189, JUN (2012), s. 60-70. ISSN 0168-9452. E-ISSN 1873-2259
Grant CEP: GA ČR(CZ) GAP501/10/0340; GA AV ČR(CZ) IAA600110507; GA AV ČR IAA600870701; GA MŠMT(CZ) LC06063; GA MŠMT(CZ) LM2010007
Grant ostatní: GA ČR(CZ) GD206/03/H137
Program: GD
Výzkumný záměr: CEZ:AV0Z60870520; CEZ:AV0Z50380511; CEZ:AV0Z50110509
Klíčová slova: Elevated CO2 * Mesophyll structure * Phenolic compounds * Photosynthesis * Picea abies * Stereological methods
Kód oboru RIV: EH - Ekologie - společenstva; ED - Fyziologie (FGU-C); EF - Botanika (UEB-Q)
Impakt faktor: 2.922, rok: 2012

Norway spruce (Picea abies L. Karst) grown under ambient (365–377 μmol (CO2) mol−1; AC) and elevated (700 μmol (CO2) mol−1; EC) CO2 concentrations within glass domes with automatically adjustable windows and on an open-air control site were studied after 8 years of treatment. The effect of EC on photosynthesis, mesophyll structure and phenolics accumulation in sun and shade needles was examined. Photosynthetic assimilation and dark respiration rates were measured gasometrically; the structural parameters of mesophyll were determined using confocal microscopy and stereological methods. The contents of total soluble phenolics and lignin were assessed spectrophotometrically, and localizations of different phenolic groups were detected histochemically on needle cross-sections. EC enhanced the light-saturated CO2 assimilation rate and reduced dark respiration in the current-year needles. No effects of CO2 enrichment on mesophyll structural parameters were observed. Similarly, the accumulation and localization of phenolics and lignin remained unaffected by EC treatment. Needles differentiated into sun and shade ecotypes in the same manner and to the same extent irrespective of CO2 treatment. Based on these results, it is apparent that the EC-induced enhancement of photosynthesis is not related to changes in the examined structural parameters of mesophyll and accumulation of phenolic compounds.
Trvalý link: http://hdl.handle.net/11104/0210503